mst 3 antibody mouse  (Santa Cruz Biotechnology)

Journal: International Journal of Molecular Sciences

Article Title: The Chronic Elevated Consumption of Hibiscus sabdariffa Linnaeus Results in Kidney Damage Associated with Excess H 2 S

doi: 10.3390/ijms27052190

Figure Lengend Snippet: Immunohistochemistry analysis of areas marked in the kidney cortex on the expression of the enzymes involved in the H 2 S pathway. Panel ( a ) = CBS, ( b ) = CSE, ( c ) = 3–MST and ( d ) = GCLC. Abbreviations: CBS = cystathionine–β–synthase, CSE = cystathionine–γ–lyase, 3–MST = 3–mercaptopyruvate, GCLC = γ-glutamylcysteine synthetase. Data are means ± SE; n = 7 each group. AU = arbitrary units.

Article Snippet: For Western blot, the antibodies were CBS antibody mouse IgG 1 κ: sc-133154, CSE antibody mouse (F-1): sc-374249, MST-3 antibody mouse (46): sc-135993, and GCLC antibody [EP13475], which were provided by Santa Cruz Biotechnology.

Techniques: Immunohistochemistry, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Chronic Elevated Consumption of Hibiscus sabdariffa Linnaeus Results in Kidney Damage Associated with Excess H 2 S

doi: 10.3390/ijms27052190

Figure Lengend Snippet: Representative Western blot images that represent the expression of the enzymes involved in the H 2 S synthesis pathway in the three experimental groups. Panel ( a ) = CBS, ( b ) = CSE, ( c ) = 3–MST and ( d ) = GCLC. Abbreviations: CBS = cystathionine–β–synthase, CSE = cystathionine–γ–lyase, 3–MST = 3–mercaptopyruvate, GCLC = γ-glutamylcysteine synthetase. Data are means ± SE; n = 7 each group.

Article Snippet: For Western blot, the antibodies were CBS antibody mouse IgG 1 κ: sc-133154, CSE antibody mouse (F-1): sc-374249, MST-3 antibody mouse (46): sc-135993, and GCLC antibody [EP13475], which were provided by Santa Cruz Biotechnology.

Techniques: Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Chronic Elevated Consumption of Hibiscus sabdariffa Linnaeus Results in Kidney Damage Associated with Excess H 2 S

doi: 10.3390/ijms27052190

Figure Lengend Snippet: Immunohistochemistry analysis of areas marked in the kidney cortex on the expression of the enzymes involved in the H 2 S pathway. Panel ( a ) = CBS, ( b ) = CSE, ( c ) = 3–MST and ( d ) = GCLC. Abbreviations: CBS = cystathionine–β–synthase, CSE = cystathionine–γ–lyase, 3–MST = 3–mercaptopyruvate, GCLC = γ-glutamylcysteine synthetase. Data are means ± SE; n = 7 each group. AU = arbitrary units.

Article Snippet: For immunohistochemistry the antibodies were CBS antibody mouse IgG 1 κ (sc-133154 Santa Cruz Biotechnology Dallas, Dallas, TX, USA), CSE antibody mouse (F-1) (sc-374249 Santa Cruz Biotechnology), MST-3 antibody mouse (48) (sc-135993 Santa Cruz Biotechnology), and GCLC antibody [EP13475] ab190685 (Abcam, Cambridge, UK).

Techniques: Immunohistochemistry, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Chronic Elevated Consumption of Hibiscus sabdariffa Linnaeus Results in Kidney Damage Associated with Excess H 2 S

doi: 10.3390/ijms27052190

Figure Lengend Snippet: Representative Western blot images that represent the expression of the enzymes involved in the H 2 S synthesis pathway in the three experimental groups. Panel ( a ) = CBS, ( b ) = CSE, ( c ) = 3–MST and ( d ) = GCLC. Abbreviations: CBS = cystathionine–β–synthase, CSE = cystathionine–γ–lyase, 3–MST = 3–mercaptopyruvate, GCLC = γ-glutamylcysteine synthetase. Data are means ± SE; n = 7 each group.

Article Snippet: For immunohistochemistry the antibodies were CBS antibody mouse IgG 1 κ (sc-133154 Santa Cruz Biotechnology Dallas, Dallas, TX, USA), CSE antibody mouse (F-1) (sc-374249 Santa Cruz Biotechnology), MST-3 antibody mouse (48) (sc-135993 Santa Cruz Biotechnology), and GCLC antibody [EP13475] ab190685 (Abcam, Cambridge, UK).

Techniques: Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Transcriptional Induction of Cystathionine γ-Lyase, a Reactive Sulfur-Producing Enzyme, by Copper Diethyldithiocarbamate in Cultured Vascular Endothelial Cells

doi: 10.3390/ijms21176053

Figure Lengend Snippet: Expression of reactive sulfur species-producing enzymes in dense and sparse vascular endothelial cells after treatment with copper diethyldithiocarbamate (Cu10). ( a ) Structure of Cu10. ( b ) Levels of cystathionine γ-lyase (CSE) protein in dense (upper panels) and sparse (lower panels) cell cultures after treatment with Cu10. The ratio to the intensity of CSE to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH); values are mean ± SE of three replicates from three independent experiments. ( c ) Levels of CSE mRNA in dense (left panel) and sparse (right panel) cell cultures after treatment with Cu10. ( d ) Levels of cystathionine β-synthase (CBS), 3-mercaptopyruvate sulfurtransferase (3-MST), and cysteinyl-tRNA synthetase (CARS2) protein (left panels) and mRNA (right panels) in dense (upper panels) and sparse (lower panels) cell cultures after treatment with Cu10. The results of the densitometric analysis are shown in . Dense cultures of bovine aortic endothelial cells were treated with Cu10 (1, 2, 5, 8, or 10 µM) for 24 h (left panels) or with Cu10 (5 µM) for 4, 8, 12, and 24 h (right panels). ○, Control, ●, Cu10 treatment. Values are mean ± SE of three technical replicates. Significant difference from corresponding control, * p < 0.05; ** p < 0.01. Sparse cultures of bovine aortic endothelial cells were treated with Cu10 (0.1, 0.2, 0.5, 0.8, or 1.0 µM) for 24 h (left panels) or with Cu10 (5 µM) for 4, 8, 12, and 24 h (right panels). ○, Control, ●, Cu10 treatment. Values are mean ± SE of three technical replicates. Significant difference from corresponding control, * p < 0.05; ** p < 0.01.

Article Snippet: Anti-MPST (3-MST) mouse monoclonal antibody (D-8), anti-CREB2 (ATF4) rabbit polyclonal antibody (C-20), and anti-lamin A/C mouse monoclonal antibody (636) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Expressing, Control